rhogap activity assay  (Cytoskeleton Inc)

Journal: International Journal of Medical Sciences

Article Title: Aberrant DNA Methylation in Esophageal Squamous Cell Carcinoma and its Clinical Implications in Systemic Chemotherapy

doi: 10.7150/ijms.109161

Figure Lengend Snippet: Hypermethylated functional genes in esophageal squamous cell carcinoma

Article Snippet: , 2007 , DLC1 , DLC1 Rho GTPase Activating Protein , Cytoskeleton organization, Ras-mediated signaling pathways, cell adhesion, cell cycle, tumor suppressor , 8p22 , 51 , .

Techniques: Functional Assay, Methylation, Migration, Control, Inhibition, Binding Assay, Activity Assay, Transduction, Cell Attachment Assay, Cell Differentiation

Journal: JACC: Basic to Translational Science

Article Title: Decreased FAM13B Expression Increases Atrial Fibrillation Susceptibility by Regulating Sodium Current and Calcium Handling

doi: 10.1016/j.jacbts.2023.05.009

Figure Lengend Snippet: FAM13B Localization and Recombinant Protein (A–D) FAM13B-GFP fusion protein expression in iCell cardiomyocytes green fluorescent protein (GFP) fluorescence showing expression in a sarcomere pattern and on the plasma membrane (A). (B) α-actinin immunofluorescence (red) showing Z-disc localization with the transfected cell surrounded by nontransfected cells. (C) Hoechst 33342 staining of nuclei. (D) Merged image, showing FAM13B-GFP expression overlaps with or is adjacent to the Z-disc marker protein α-actinin; ×60 objective lens, scale bar shows 20 μm. (E) rhFAM13B purification. 6 Histidine (6xHis)-maltose binding protein (MBP)-hFAM13 fusion protein in bacterial lysate was eluted from an amylose column (left panel), cleaved with 6xHis-Tobacco Etch Virus (TEV) protease releasing the rhFAM13B from the 6xHis-MBP affinity tags (center panel), and purified by flow through in the Ni resin column (right panel). (F) Rho GTPase-activating protein (RhoGAP) activity assay (in singleton assay) showing background GTPase activity of the p50 RhoGAP alone (striped bar) and for each of the 4 Rho proteins alone (white bars), whereas p50 RhoGAP increased the GTPase activity of 3 of 4 Rho proteins (black bars). In contrast, rhFAM13B did not increase GTPase activity for any of the 4 Rho proteins (gray bars).

Article Snippet: To determine if recombinant human FAM13B (rhFAM13B) had RhoGAP activity, a commercially available RhoGAP Assay kit (Cytoskeleton, Cat#BK105) was used.

Techniques: Recombinant, Expressing, Fluorescence, Membrane, Immunofluorescence, Transfection, Staining, Marker, Purification, Binding Assay, Virus, Activity Assay

Journal: PLoS ONE

Article Title: Gene Expression Patterns in Peripheral Blood Correlate with the Extent of Coronary Artery Disease

doi: 10.1371/journal.pone.0007037

Figure Lengend Snippet: List of 160-gene model predictive of the extent of coronary artery disease.

Article Snippet: ARHGAP4 , 204425_at , 39649_at , Rho GTPase activating protein 4 , Cytoskeleton , 0.23.

Techniques: Binding Assay, RNA Binding Assay, Coagulation, Activation Assay, Cell Differentiation, Histone Deacetylase Assay